Summary
Type: SPE
Application #: 305540
Catalog#: 730425 - SPE Cartridges, Chromabond SA, 6 ml, 500 mg, 30/pk
Detection:
Conditions: Column conditioning: 2 x 3 mL methanol, then 2 x 3 mL 3 % acetic acid
(Do not let run the column dry.)
Sample aspiration: 50 mL of prepared sample solution is applied to the column by help of reservoir column (REF 730381)
Washing: reservoir and beaker are twice rinsed with 2 mL 3 % acetic acid; wash solution is applied to the column
Drying: suck for 30 s
Elution: 3 x 1 mL of 0.1 mol/L citrate buffer – methanol (70:30, v/v) + 3 % acetic acid (w/v) into a 5 mL volumetric flask (Between the 1 mL steps suck the column dry.)
Dilution: fill-up with bidistilled water to the mark of flask
Authors: SPE department
Source: Macherey-Nagel, Germany 2012